This thread has some great information for those considering the option of gaining root access on their Android devices. It definitely takes some research and consideration because, without knowing why you're doing it in the first place, there is no way to actually benefit fully from having it done. Even then, the benefits probably won't live up to the hype that a lot of members of the Android community seem to promise.
In the end, my primary motivation to root my TF101 was for AdFree Android and SetCPU. I like the cleaner look of apps without ads (though buying the paid version is a completely viable alternative, and it goes a long way to showing appreciation to the developers) and I had hopes of squeezing a little bit more battery life by tweaking the processor settings. From there, I decided to try a custom ROM (Revolver) in hopes of better performance. Admittedly, the stock configuration was no slouch. I can't actually say with concrete proof whether or not my Transformer is faster post-flashing. What I do get out of it, however, is the psychological aspect of feeling like it has to have gotten faster. For some people, myself included, that may be enough of a reward.
I would be completely fine running a stock Android install without root access. We are fortunate that Asus is supporting our tablets with an Ice Cream Sandwich update to keep things current. I could definitely see the advantage of rooting and flashing custom ROMs on devices that don't get that kind of prolonged support. In our case, new Android updates isn't that big of a game changer, for now. Ice Cream Sandwich is another story altogether. I'm curious if it will live up to the hype or if it's just an instance of the "newer has to be better and it will fix everything" mentality. Regardless, I'm excited to try it out because newer has to be better!
I'm happily rooted because I put the time in to consider all of the benefits and weigh them against the possible risks. In the end, I ruled that it would be worth it for a few key reasons. I was fortunate enough to not encounter any issues and I look forward to getting back to just using my tablet to do the tasks that it was meant to do.